Neutral Red Assay for Measurement of Quantitative Vero Cell Cytotoxicity
نویسندگان
چکیده
منابع مشابه
Neutral red assay for measurement of quantitative vero cell cytotoxicity.
A neutral red assay involving Vero cells was used to quantitate the cytotoxic activity of verotoxins (VT) produced by Escherichia coli and to investigate changes in titer caused by altering the composition of the cell culture medium. Three variations on medium 199 were investigated: one involved supplementing the medium with 5% fetal bovine serum (FBS), a second was the use of serum-free (SF) m...
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Acute oral toxicity is currently being assessed by a suite of refinement test methods based on thetraditional LD50 lethality test and is, besides skin sensitisation, the only remaining animal test requiredunder REACH Annex VII. In view of assessing the use of alternatives for this health endpoint, EURL ECVAMconducted a study on the 3T3 Neutral Red Uptake cytotoxicity test method add...
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A rapid assay which measured the biological activity of Clostridium perfringens enterotoxin was developed. The method involved the rapid killing of Vero cells by enterotoxin produced by C. perfringens grown in Duncan and Strong sporulation medium. Serial dilutions of toxin were added to Vero cells either in suspension or grown as monolayers in wells of a 96-well cell tissue culture cluster plat...
متن کاملCytotoxicity evaluation of Dionysia revoluta Boiss. on VERO and Hep-2c cell lines
Background and objectives: Evaluating the safety of pharmaceutical products by in vitro methods like cell culture, and in vivo tests on animals, is one of the regulatory steps in development of drugs which is called the preclinical stage. If acceptable results are obtained, the drug can be used in clinical studies. In this study, we have investigated the cytot...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1993
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.59.6.1981-1983.1993